Redesign of ultrasensitive and robust RecA gene circuit to sense DNA damage

Response of recA-dependent operons to different DNA damage signals.

The responses of three recA-dependent operons (recA, lambda, and phi 80), with three different repressors, to five DNA damage treatments were compared. Each operon shows a unique induction onset time constant over a wide dose range. However, the extent of response is variable within the same dose range. Individual Escherichia coli cells show a graded SOS response to different levels of DNA dama...

OGG1 DNA Repair Gene Polymorphism As a Biomarker of Oxidative and Genotoxic DNA Damage

Background: Single nucleotide polymorphisms in 8-oxoguanine DNA glycosylase-1 (OGG1) gene modulates DNA repair capacity and functions as one of the first lines of protective mechanisms against 8-hydroxy-2’-deoxyguanosine (8-OHdG) mutagenicity. OGG1-Cys326 gene polymorphism may decrease DNA repair function, causing oxidative stress due to higher oxidative DNA damage. The main purpose of this stu...

The mycobacterium-specific gene Rv2719c is DNA damage inducible independently of RecA.

The mycobacterium-specific gene Rv2719c was found to be expressed primarily from a promoter that was clearly DNA damage inducible independently of RecA. Upstream of the transcriptional start site for this promoter, sequence motifs resembling those observed previously at the RecA-independent, DNA damage-inducible recA promoter were identified, and the -10 motif was demonstrated by mutational ana...

Cloning and DNA sequence of a mycoplasmal recA gene.

Mycoplasmas are wall-less prokaryotes phylogenetically related to gram-positive bacteria. In order to investigate DNA recombination in these organisms, we have cloned the recA gene from the mycoplasma Acholeplasma laidlawii. DNA sequence data indicate extensive homology between the A. laidlawii recA gene and recA genes from other bacteria, particularly Bacillus subtilis. The recA sequences from...

Radiation track and DNA damage